Studies on Phytochemicals, Antioxidant and Cytotoxicity Effect of Hybanthus Enneaspermus

Objectives: To determine the qualitative and quantitative phytochemical and evaluate radical scavenging, antioxidant and cytotoxicity effect of Hybanthus enneaspermus. Methods: The phytochemical of H.enneaspermus was screened qualitative and quantitative with HPTLC and FT-IR. Radical scavenging effect of different concentrations of hydroethanolic extract of H. enneaspermus (HEEHE) was evaluated by DPPH, nitric oxide, hydroxyl and reducing power. The antioxidant potential was evaluated using Ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods and cytotoxicity was determined in Hep2 cell lines. Results: Qualitative analysis of HEEHE showed the presence of alkaloids, flavonoids, carbohydrates, protein, phenol, steroid, tannin, glycosides and terpenoids. The results expressed higher amount of glycosides (45mg/g), tannins (42mg/g) and Iron (4.6mg/g) in HEEHE. 500μg/ml of HEEHE demonstrated significant radical scavenging and cytotoxicity effect. Lycopene exhibited significant amount (0.832g) among the different antioxidants. IR spectra specified the presence of asymmetric and aromatic compounds. HPTLC chromatogram showed the presence of flavonoid and glycosides in H.enneaspermus. Conclusion: This study concludes therapeutic potential of H.enneaspermus, will be used for clinical trials..